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mesenchymal stem cell growth kit components  (ATCC)


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    ATCC mesenchymal stem cell growth kit components
    Mesenchymal Stem Cell Growth Kit Components, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mesenchymal+stem+cell+growth+kit/Mesenchymal+Stem+Cell+Growth+Kit+for+Adipose+and+Umbilical-derived+MSCs+-+Low+Serum/pmc13185996-184-21-27
    Average 95 stars, based on 136 article reviews
    mesenchymal stem cell growth kit components - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: Lipoprotein Association Fluorometry (LAF) as a Semi‐Quantitative Characterization Tool to Assess Extracellular Vesicle‐Lipoprotein Binding
    Article Snippet: NK‐92 cells were further supplemented with 300 IU/mL recombinant interleukin‐2 (IL‐2, PeproTech, 200‐02). .. ASC52telo, human hTERT immortalized adipose‐derived mesenchymal stromal cells (MSC, ATCC, SCRC‐4000) were cultured in mesenchymal stem cell basal medium for adipose, umbilical and bone marrow‐derived mesenchymal stem/stromal cells (ATCC, PCS‐500‐030) and supplemented with mesenchymal stem cell growth kit for adipose and umbilical‐derived mesenchymal stem/stromal cells—low serum (ATCC, PCS‐500‐040). .. All cells were incubated at 37°C in 5% CO 2, and cell viability was determined by trypan blue exclusion (Gibco, 15250061).

    Article Title: Engineered tumor-tropic mesenchymal stem cells as targeted therapeutic delivery systems for refractory Ovarian cancer.
    Article Snippet: .. ASC-shCE2:yCD cells were cultured in mesenchymal stem cell basal medium (ATCC, Catalog # PCS-500-030) supplemented with mesenchymal stem cell growth kit (ATCC, Catalog # PCS-500-040) at 37 ◦C and 5 % CO2 as described previously [15]. .. The drugs and prodrugs used in this study were purchased from Sigma-Aldrich (St. Louis, MO, United States).

    Article Title: Modulation of epileptogenesis through transplantation of human mesenchymal stem cells with or without GDNF release
    Article Snippet: For FACS, the transduced cells were excited with a yellow‒green laser at 561 nm, and mCherry fluorescence emission was detected by a 610/20 nm detector via a FACSAria Fusion system (BD Biosciences, Franklin Lakes, NJ). .. The cells were cultured in T75 flasks in Mesenchymal Stem Cell Basal Medium (ATCC PCS-500-030, ATCC, Manassas, VA, USA) supplemented with a Mesenchymal Stem Cell Growth Kit (ATCC PCS-500-040, ATCC, Manassas, VA, USA), penicillin‒streptomycin (10 units/ml, Gibco, Waltham, MA, USA) and amphotericin B (25 ng/ml, Gibco, Waltham, MA, USA). ..

    Article Title: Method of fabricating a magnetic deformable machine and deformable 3D magnetic machine
    Article Snippet: .. Human adipose-derived mesenchymal stem cells 120 (haMSCs, ATCC) were cultured in a humidified, 37° C., 5% CO2 environment using 75 cm2 polystyrene cell culture flasks containing MSC basal medium (ATCC) supplemented with mesenchymal stem cell growth kit for low serum (ATCC), 2% fetal bovine serum (FBS, Gibco), penicillin (25 UI mL−1) and streptomycin (25 μg mL−1). .. Cell were split before they reached to 80% confluence by 0.25% Tyrpin-EDTA (Gibco) solution.

    Article Title: Targeting miR-184 to Enhance Corneal Epithelial Wound Healing: In Vivo Knockout, Antisense Inhibition, and Exosome-Mediated Delivery.
    Article Snippet: To evaluate the therapeutic efficacy of exosomes loaded with LNA-anti-miR-184 on CEWH, human adipose-derived Downloaded from iovs.arvojournals.org on 11/07/2025 mesenchymal stem cells (hMSCs; PCS-500-011; American Type Culture Collection, Manassas, VA, USA) were transfected with either 100nM LNA-anti-miR-184 (GenePharma Co., Ltd., Shanghai, China), or LNA-anti-NC, using Lipofectamine RNAiMAX (Thermo Fisher Scientific). .. The hMSCs were cultured in Mesenchymal Stem Cell Basal Medium (PCS-500-030; American Type Culture Collection) supplemented with the Mesenchymal Stem Cell Growth Kit (PCS500-040; American Type Culture Collection). .. Six hours after transfection, the standard serum was replaced with exosome-depleted fetal bovine serum (EXO-FBS-50A-1; System Biosciences, Palo Alto, CA, USA), and the cells were further cultured for 48 hours.

    Article Title: Targeting miR-184 to Enhance Corneal Epithelial Wound Healing: In Vivo Knockout, Antisense Inhibition, and Exosome-Mediated Delivery
    Article Snippet: To evaluate the therapeutic efficacy of exosomes loaded with LNA-anti-miR-184 on CEWH, human adipose-derived mesenchymal stem cells (hMSCs; PCS-500-011; American Type Culture Collection, Manassas, VA, USA) were transfected with either 100nM LNA-anti-miR-184 (GenePharma Co., Ltd., Shanghai, China), or LNA-anti-NC, using Lipofectamine RNAiMAX (Thermo Fisher Scientific). .. The hMSCs were cultured in Mesenchymal Stem Cell Basal Medium (PCS-500-030; American Type Culture Collection) supplemented with the Mesenchymal Stem Cell Growth Kit (PCS-500-040; American Type Culture Collection). .. Six hours after transfection, the standard serum was replaced with exosome-depleted fetal bovine serum (EXO-FBS-50A-1; System Biosciences, Palo Alto, CA, USA), and the cells were further cultured for 48 hours.

    Reverse Transcription:

    Article Title: Fixed-Bed Bioreactor Culture Enhances Yield and Reparative Properties of hTERT Mesenchymal Stem Cell Extracellular Vesicles.
    Article Snippet: All cell lines were obtained from ATCC (Manassas, VA, USA). .. Human telomerase reverse transcriptase (hTERT) mesenchymal stem cells (MSCs) (ATCC® SCRC-4000TM) were maintained in Mesenchymal Stem Cell Basal Medium (ATCC® PCS-500-030TM) supplemented with Mesenchymal Stem Cell Growth Kit (ATCC® PCS-500-040TM). .. Normal human primary dermal fibroblasts (ATCC® PCS-201-012TM) were maintained in Fibroblast Basal Medium (ATCC® PCS-201-030TM) supplemented with Fibroblast Growth Kit (ATCC® PCS-201-040TM).

    Article Title: Fixed-Bed Bioreactor Culture Enhances Yield and Reparative Properties of hTERT Mesenchymal Stem Cell Extracellular Vesicles
    Article Snippet: All cell lines were obtained from ATCC (Manassas, VA, USA). .. Human telomerase reverse transcriptase (hTERT) mesenchymal stem cells (MSCs) (ATCC ® SCRC-4000TM) were maintained in Mesenchymal Stem Cell Basal Medium (ATCC ® PCS-500-030TM) supplemented with Mesenchymal Stem Cell Growth Kit (ATCC ® PCS-500-040TM). .. Normal human primary dermal fibroblasts (ATCC ® PCS-201-012TM) were maintained in Fibroblast Basal Medium (ATCC ® PCS-201-030TM) supplemented with Fibroblast Growth Kit (ATCC ® PCS-201-040TM).



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    Effects of GPE on human mesenchymal stem viability. BMSC and AdMSC were exposed at increasing concentrations of GPE (1, 5, 10 and 25 µg GAE/mL) for 24 h and cell viability was evaluated by MTT assay. Data are shown as mean ± SD ( n = 5); * p < 0.05 versus untreated control cells (CTR).

    Journal: Biology

    Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

    doi: 10.3390/biology15090719

    Figure Lengend Snippet: Effects of GPE on human mesenchymal stem viability. BMSC and AdMSC were exposed at increasing concentrations of GPE (1, 5, 10 and 25 µg GAE/mL) for 24 h and cell viability was evaluated by MTT assay. Data are shown as mean ± SD ( n = 5); * p < 0.05 versus untreated control cells (CTR).

    Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

    Techniques: MTT Assay, Control